Column Chromatography of Basic Antibiotics on Column-lite
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Phosphorus assay in column chromatography.
Malerials-30 per cent hydrogen peroxide (Baker and Adamson, phosphorus-free) and the ammonium molybdate, (NHJ6lMo7024.4Hz0, wereused. The Fiske and SubbaRow (I) reagent was prepared by adding 0.5 gm. of purified (1) 1-amino-2naphthol-4-sulfonic acid (Eastman Organic Chemicals) with mechanical stirring to 200 ml. of freshly prepared 15 per cent sodium bisulfite (anhydrous), followed by 1.0 gm. o...
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ژورنال
عنوان ژورنال: Journal of the agricultural chemical society of Japan
سال: 1974
ISSN: 0002-1407,1883-6844
DOI: 10.1271/nogeikagaku1924.48.397